Occurrence of P-Flavin Binding Protein in Vibrio fischeri and Properties of the Protein.

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概要

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• In previous studies involving Photobacterium species we proposed that (i) P-flavin is the product of luciferase, (ii) the physiological function of the lux operon is not to produce light but to produce FP390 (luxF protein), including its prosthetic group, P-flavin, and (iii) FP390 reactivates oxidatively inactivated cobalamin-dependent methionine synthase similar to flavodoxin but at relatively high ionic strength. It seems difficult to extend this idea to all luminous bacteria because the luxF gene is not present in the lux operon in Vibrio or Xenorhabdus. But we predicted that a luciferase fragment which binds P-flavin should function like FP390 in these species. In this study, we isolated P-flavin binding protein from Vibrio fischeri ATCC 7744. The obtained protein was a modified luciferase as expected, in which the β-subunit was intact but about 25 amino acid residues at the C-terminus of the α-subunit were deleted and the prosthetic group was the fully reduced P-flavin. These results strongly support that the physiological function of the lux operon is as described above even in luminous bacteria other than Photobacterium species. We propose that chromophore B reported by Tu and Hastings [Tu, S. -C. and Hastings, J. W. (1975) Biochemistry 14, 1975-1980] is the reduced P-flavin.

• 社団法人 日本生化学会の論文

著者

• KASAI Sabu

  Department of Bioapplied Chemistry, Faculty of Engineering, Osaka City University

• Kasai Sabu

  Department Of Applied Chemistry And Bioapplied Chemistry Graduate School Of Engineering Osaka City U

関連論文

• Crystal Structure Determination of a Flavoprotein FP_ from a Luminescent Bacterium, Photobacterium phosphoreum^1
• Freshwater Bioluminescence in Vibrio albensis (Vibrio cholerae biovar albensis) NCIMB 41 Is Caused by a Two-Nucleotide Deletion in luxO
• Occurrence of P-Flavin Binding Protein in Vibrio fischeri and Properties of the Protein
• Lateral Transfer of the lux Gene Cluster
• Recovery of the Ultrastructure of Jejunal Absorptive Cells and Absorption of Nutrients in Starved Then Re-fed Rats.
• Occurrence of P-Flavin Binding Protein in Vibrio fischeri and Properties of the Protein.

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