Identification and Characterization of Ligands for L-Selectin in the Kidney. III. Characterization of L-Selectin Reactive Heparan Sulfate Proteoglycansl.

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概要

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• L-Selectin, a leukocyte adhesion molecule, mediates leukocyte rolling on the endothelium and plays a critical role in leukocyte recruitment at inflammatory sites as well as in lymphocyte homing. We have previously shown that L-selectin reactive chondroitin sulfate and heparan sulfate proteoglycans (HSPGs) are both expressed in the distal tubules of the kidney and that versican is one of the chondroitin sulfate-type ligands. In the present study, we characterized the heparan sulfate-type ligand(s) in more detail. The molecular sizes of HSPGs were approximately 600 kDa with core protein sizes of 160 and 180 kDa. Western blotting analysis showed that L-selectin reactive HSPGs were neither agrin nor perlecan, major basement membrane HSPGs in the kidney. The binding to L-selectin was mediated by the lectin domain of L-selectin in a Ca2+-dependent manner and required heparan sulfate side chains, but not sialic acid. To our knowledge, this is the first biochemical characterization of the L-selectin reactive heparan sulfate proteoglycan(s) in the distal tubules of the kidney.

• 社団法人 日本生化学会の論文

著者

• Kawashima Hiroto

  Department Of Bioregulation Biomedical Research Center Osaka University Graduate School Of Medicine

• Miyasaka Masayuki

  Department Of Bioregulation Biomedical Research Center Osada University Medical School

• Li Yong-fei

  Department Of Bioregulation Biomedical Research Center Osaka University Medical School

• WATANABE Norifumi

  Department of Bioregulation, Biomedical Research Center, Osaka University Medical School

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