Structure and Distribution of N-Glycans on the S7-Allele Stylar Self-Incompatibility Ribonuclease of Nicotiana alatal.

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概要

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• S-RNases are the stylar products of the self-incompatibility (S)-locus in solanaceous plants (including Nicotiana alata), and as such, are involved in the prevention of self-pollination. All cDNA sequences of S-RNase products of functional S-alleles contain potential N-glycosylation sites, with one site being conserved in all cases, suggesting that N-glycosylation is important in self-incompatibility. In this study, we report on the structure and localization of the N-glycans on the S7-allele RNase of N. alata. A total of nine N-glycans, belonging to the high-mannose- and xylosylated hybrid-classes, were identified and characterized by a combination of electrospray-ionization mass-spectrometry (ESI-MS), 1H-NMR spectroscopy, and methylation analyses. The glycosylation pattern of individual glycosylation sites was determined by ESI-MS of the glycans released from isolated chymotryptic glycopeptides. All three N-glycosylation sites showed microhetero-geneity and each had a unique complement of N-glycans. The N-glycosylation pattern of the S7-RNase is significantly different to those of the S1- and S2-RNases.

• 社団法人 日本生化学会の論文

著者

• Oxley David

  Plant Cell Biology Research Centre School Of Botany University Of Melbourne

• Craik David

  Victorian College Of Pharmacy

• MUNRO Sharon

  Victorian College of Pharmacy

• Bacic Antony

  Plant Cell Biology Research Centre And Australian Centre For Plant Functional Genomics School Of Bot

関連論文

• Structure and Distribution of N-Glycans on the S_7-Allele Stylar Self-Incompatibility Ribonuclease of Nicotiana alata^1
• Aberrant Cell Expansion in the elongation Mutants of Barley
• 寄生虫である原虫(鞭毛虫類)リーシュマニアのムチン様プロテオホスフォグリカン
• Structure and Distribution of N-Glycans on the S7-Allele Stylar Self-Incompatibility Ribonuclease of Nicotiana alatal.

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