Diversity of Capsular Polysaccharide Synthesis Gene Clusters in Streptococcus pneumoniae.

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概要

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• Streptococcus pneumoniae comprises 90 serotypes, each one having its own specific polysaccharide capsule. In order to explore the diversity of capsular polysaccharide synthesis (cps) gene clusters in S. pneumoniae, we performed cross-hybridizations between the 12 cps genes of S. pneumoniae serotype 14 and chromosomal DNA of 26 strains comprising 26 different capsule types. Large variations in the hybridization patterns were observed. The genes cps14A to cps14D are conserved in most serotypes. Sequences homologous to cps14I to cps14L were only observed in the four types of serogroup 15, which all have a capsule structure similar to that of type 14. By using a cps14E knock-out construct, cpsE mutants of the pneumococcal types 9N, 13, and 15B were obtained. These mutants were unencapsulated and showed reduced glycosyltransferase activity, indicating that the pneumococcal types 9N, 13, and 15B express a glucosyl-1-phosphate transferase which is homologous to Cps14E. Glycosyltransferase assays showed that among 21 pneumococcal types which contain glucose in the core of their capsule polysaccharide, 19 types express glucosyl-1-phosphate transferase activity. However, not all of these types hybridized strongly with Cps14E, the type 14 glucosyl-1-phosphate transferase gene. Thus, pneumococci possess glucosyltransferase genes distinct from cps14E, but encoding enzymes with identical activity. All serotypes which synthesized lipid-linked lactose intermediates in glycosyltransferase activity assays (type 11B, 13, 15F, 15A, 15B, 15C) hybridized with cps14G. This gene encodes a galactosyltransferase which catalyzes the addition of 1, 4-linked β-galactose to lipid-linked glucose. The cps14G homologues in type 11B, 13, 15F, 15A, 15B, and 15C may encode a similar β-galactosyltransferase activity as cps14G in type 14.

• 社団法人 日本生化学会の論文

著者

• Kolkman Marc

  Department Of Bacteriology Institute Of Infectious Diseases And Immunology School Of Veterinary Medi

• Nuijten Piet

  Department of Bacteriology, Institute of Infectious Diseases and Immunology, School of Veterinary Medicine, Universiteit Utrecht

• Zeijst Bernard

  Department of Bacteriology, Institute of Infectious Diseases and Immunology, School of Veterinary Medicine, University Utrecht

関連論文

• Diversity of Capsular Polysaccharide Synthesis Gene Clusters in Streptococcus pneumoniae^1
• Diversity of Capsular Polysaccharide Synthesis Gene Clusters in Streptococcus pneumoniae.

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