Characterization of Yeast sar1 Temperature-Sensitive Mutants, Which Are Defective in Protein Transport from the Endoplasmic Reticuluml.
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概要
- 論文の詳細を見る
SAR1 encodes a low molecular weight GTPase that is essential in the early process of vesicular transport in the secretory pathway. By random and site-directed mutagenesis of the SAR1 gene, we have obtained three temperature-sensitive mutants, N132I, E112K, and D32G. They all show a defect in transport from the endoplasmic reticulum to the Golgi apparatus, and accumulate endoplasmic reticulum membranes at the restrictive temperature. This is consistent with our previous observations in vivo on a galactose-shutoff mutant as well as the in vitro results, and provides powerful tools for further genetic analyses.
- 社団法人 日本生化学会の論文
著者
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Nakano Akihiko
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Oka Toshihiko
Departemnt Of Organic Chemistry And Biochemistry Institute Of Scientific And Iindustrial Research Os
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Hirata Aiko
Institute Of Applied Microbiology The University Of Tokyo
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Yamanushi Tomoko
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Oka Toshihiko
Department of Biological Sciences, Graduate School of Science
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