Cross Combinations Giving Improved Hybrid Fertility Among Rice Varieties for Effective Application of Genetic Analysis.
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概要
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To find parental varieties suitable for detecting quantitative trait loci (QTL) associated with regeneration ability of rice seed callus, varieties showing high F<SUB>1</SUB> fertility when crossed with both Japonica and Indica testers were screened using 34 varieties originating, mainly, from Assam, India. In rice, many polymorphic markers can be obtained between Japonica and Indica, however, their cross combination often shows F<SUB>1</SUB> sterility. In detecting QTL for regeneration ability, population size is small to enable efficient evaluation with regeneration ability. We selected parental varieties with high F<SUB>1</SUB> fertility to increase precision of QTL analysis by avoiding segregation ratio deviating from the theoretical value. Varieties originating from Assam were used because they included vide variation, which would include varieties we screened for. Each variety was pollinated with a Japonica and Indica tester, and the F<SUB>1</SUB> female and male gamete fertility was evaluated for seed and pollen fertility. Seed fertility of all the varieties and testers was more than 80%, and pollen fertility was more than 85%. These values were used as criteria for determirLing "high" F<SUB>1</SUB> fertility. Seventeen out of 33 varieties used gave high F<SUB>1</SUB> seed fertility in each cross with both Japonica and Indica testers. Eight out of 32 varieties showed high F<SUB>1</SUB> pollen fertility with both testers. Pollen diameters of 12 varieties and their F<SUB>l</SUB> progeny were measured, as an additional index of the actual fertility of the male gamete, and this was found to be a useful indication of the actual fertility of the male gamete. Six varieties, namely, ARC5193, ARC5198, Akula, Calotoc, CP-SLO, and ARC5184, showed high F<SUB>l</SUB> seed and pollen fertility when crossed with Japonica and Indica testers. These varieties are considered to be the good candidates for parental varieties of hybrid populations for QTL analysis.
- 日本育種学会の論文
日本育種学会 | 論文
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