Identification and Evaluation of Turnip Mosaic Virus(TuMV) Resistance Gene in Kale Monosomic Addition Lines of Radish.
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概要
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TuMV resistance in seven types of monosomic addition Introduction lines of radish with kale chromosomes (a - g t, ypes, 2n = 19, B5) was surveyed by scoring the symptoms after the mechanical inoculation. The level of the resistance was also evaluated by ELISA in five f-type addition lirLes (B<SUB>7</SUB>, B<SUB>8</SUB>, B<SUB>1</SUB>F<SUB>1</SUB> and B<SUB>2</SUB>F<SUB>1</SUB>) obtained by crossing f-type plants (2n=19, B<SUB>5</SUB>) with five radish cultivars. Among the seven types of monosomic addition lines, the f-type line rarely showed de-tectable symptoms. The scores of the symptoms were 0.1 in f-type line, 1.6 to 2.7 in the revertants (2n=18) and 2.4 in the radrsh cultrvar 'Shogoin', respectively. The f-type plants in the subsequent generation (B6) derived from the f-type addition lines by backcrossing with the radish cultivar (Shogoin' gave a score close to that of the f-type plants (B<SUB>5</SUB>). These results suggest that the gene (s) for TuMV resistance may be located on the added f-chromosome of kale and that this trait was transmitted dominantly to the next generation along with the transmission of the f-chromo-some
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