Plant regeneration from Ipomoea triloba L. protoplasts.
スポンサーリンク
概要
- 論文の詳細を見る
Plants were regenerated from protoplasts of Ipomoea triloba L., one of the related species of sweet potato (I. batatas (L.) LAM.) at a high frequency. Protoplasts were isolated from in vitro-grown plants of I. triloba L. and then cultured in a modified liquid MURASHIGE and SKOOG (MS) medium containing 0.5mg/l 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 1.0mg/l kinetin. First cell division occurred within 3-4 days. After 2 weeks protoplast plating efficiency was up to about 60%. After 12 weeks protoplast-derlved calli up to 2 mm in diameter were transferred onto solid MS medium supplemented with 0.2mg/l 2, 4-D for the proliferation. Three weeks after transference, they were further transferred onto MS medium supplemented with 0- 0.1mg/l 3-indoleacetic acid (IAA) and 1.0-5.0mg/l 6-benzylaminopurine (BAP) (regeneration medium) and after 2 weeks started to form adventitious roots. Subsequently, the calli were further cultured on MS medium without plant growth regulators and started to regenerate shoots 5 days after transference. When 2.0mg/l BAP was added to the regeneration medium, the regeneration frequency of protoplast-derived calli up to 36.7% was achieved. It was found that (0.2mg/l) 2, 4-D included in the proliferation medium played an important role in promoting shoot regeneration. After the regenerated shoots were transferred onto fresh MS medium without plant growth regulators, they developed into whole plants which were grown to maturity in pots with vermiculite.
- 日本育種学会の論文
日本育種学会 | 論文
- 根粒超着生変異品種作系4号の親子鑑定
- 物性測定のための米一粒による炊飯方法の開発
- いもち病抵抗性に関する同質遺伝子系統「コシヒカリ新潟BL」のDNAマーカーによる品種判別
- 巨大胚水稲新品種「はいみのり」の育成
- イネ低アミロース系統の登熟気温による胚乳アミロース含有率変動の系統間差異