Breeding Study on the Tea Plant "Leaf smear method" and the method of early identification of the Variated plant treated with colchicine solution. as a breeding technique of polyploid plants

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One of the important problems in the breeding of polyploid plants is the early identification of chromosome doubling plants induced by the colchicine treatment. In this paper the authers report the study of the "Leaf smear method" which applied to the investigation of Solanum plants. and find this method will be effective in the study of tea plants. The o;tline of the autllors investigation is follows :(1) Materials : The suitable specimen will be find in the parenchymatous layer of the Ist spreding leaf, on the young leaf bud. (2) Microscopic management : Fix the specimen about 5-10 minutes in the HCI solution (1:10)and wash in water. Smear it slightly on the slide glass, stain it with aceto-carmine solution or aceto-orcein solution (1-2%), put the cover glass on it, and crush the specimen carefully with the finger by holding it down under the coverglass, then we probably find the suitable cells which present the mitotic metaphase chromosome behavior.
日本育種学会の論文