Production of Interspecific Hybrids in Brassica campestris × B. oleracea by Culture in vitro of Excised Ovaries : II. Development of Excised Ovaries on Various Culture Media

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Studies on development in vitro of excised ovaries in the cross of B. caprrpestris L. ssp. chileensis (L.) MAKlNO cv. Seppaku-taina X B. oleracea L. var. acephala DC. cv. Portugal-kale were carried out on various culture media and on various sucrose concen-trations. Ovaries excised 4 days after pollination were cultured ile vitro. In the experiment of various cuture media, the media used were solution of minerals and vitamins of WHITE (1963), NITSCH (1951), HELLER (1953), NITSCH and NITSCH (1969), and MURASHIGE and SKOOG (1962) respectively. The media of NITSCH (1951) and HELLER (1953) were added with WHITE'S vitarnins. A11 media were further added with 50g/l of sucrose and 8g/l of agar. Many seeds and well developed naked-embryos protruded from undeveloped seed coat were obtained in the capsules examined. Production rate (Number of seeds and naked-embryos/Number of capsules examined) of hybrid plants was better in NITSCH and NlTSCH's, and MURASHIGE and SKOOG's minerals than in other culture minerals (WHITE, NITSCH and HELLER). In the experiment of various sucrose concentrations, the medium was composed of WHITE'S medium (1963) supplemented with 8g/l, of agar. Concentration of sucrose in the medium was 50g/l, 70g/l, 90g/l, IIOg/l, 130g/l, 150g/l, 170g/l, 190g/l and 210g/l respectively. High concentration of sucrose in the medium was not harmful in produc-tion of the hybrid plants by culture in vitro of excised ovaries.
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