Analysis of Human Ouabainlike Compound by Micellar Electrokinetic Chromatography
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概要
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In this preliminary study we have optimised micellar electrokinetic chromatography (MEKC, a form of capillary electrophoresis) to enable the chromatographic and spectral characteristics of human ouabainlike compound (OLC) to be investigated. Sera from fifty patients were combined to form a pool (100ml) whilst urine (92.5ml) was obtained from a normal healthy volunteer. Both samples were initially concentrated and partially purified by solid phase extraction before further purification by sequential HPLC separations. Final volumes for both extracts were 100μl. MEKC was performed on a HP3D CE instrument with voltage set at 20KV, capillary temperature at 20°C, injection time 4s, sample volume 10nl, with detection by photodiode array. A compound was found in both serum and urine that had similar elution and spectral characteristics to authentic ouabain. We conclude that MEKC is potentially a useful tool for the analysis of human OLC. (Hypertens Res 2000; 23 Suppl: S29-S32)
- 日本高血圧学会の論文
日本高血圧学会 | 論文
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