Applicability of Environmental Microorganisms to Alkaline Single Cell Gel Electrophoresis (Comet) Assay.
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概要
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Possibilities of the alkaline single cell gel electrophoresis (comet) assay were experimentally investigated, using 5 kinds of environmental microorganisms i.e. <I>Euglena gracilis, Chlamydomonas pulsatilla, Saccharomyces cerevisiae, Monas guttula</I> and <I>Tetraphymena pyriformis.</I> Consequently, applicability of <I>E.gracilis</I> to comet assay was found and its most suitable modes of operation were determined to be cell lysis for 5 min by 0.01%-sodium dodecyl sulfate (SDS) solution, unwinding for 20 min and electrophoresis (current: 300mA, voltage: 25 V) for 20 min, respectively. Tail length and Tail moment have been confirmed to be important as evaluation indices of comet assay through the experiments of γ-irradiation.
- 日本水処理生物学会の論文
日本水処理生物学会 | 論文
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