Detection of an Immunoreactive Proteinase in the Culture Media from Siborothrix schenckii by Enzyme-Linked Immunosorbent Assay.
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概要
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A preliminary in vitro study was performed for the purpose of detecting immunoreactive proteinase. A polyclonal antibody against S. schenckii proteinase (II) was prepared in a rabbit. A highly reactive lot was isolated and its sensitivity and specificity examined by enzyme-linked immunosorbent assay (ELISA). Using this antibody, 0.1μg/ml proteinase was detected without any cross-reactivity with Candida albicans proteinase. Proteinase activity and the amount of immunoreactive proteinase were well correlated with fungal growth in a liquid culture medium containing albumin as the sole nitrogen source. Maximal amount of immunoreactive proteinase was observed at a level of 25μg/ml on the 6th culture day. This antibody did not detect immunoreactive proteinase from C. albicans though high proteinase activity was observed. These in vitro data suggest that detection of immunoreactive proteinase in the serum of sporothricosis patients is possible.
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