Study of Entomophthora exitialis ATCC 32890 and its Artificial Mutant No. 1 on the Pathogenicity.
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The pathogenicity of two strains of Entomophthora exitialis (ATCC 32890 and its artificial mutant No. 1), one strain of Conidiobolus heterosporus (TACC 12941) and two strains of Basidiobolus haptosporus (MMC 68 and MMC 69) was examined experimentally using normal and diabetic BALB/c male mice (5-6 weeks, 20-25g).Both normal and diabetic mice were inoculated intravenously with each fungus suspension consisting of conidia and zygospores. The mice were then sacrificed at predetermined intervals. Portions of each organ were inoculated on potato dextrose agar plates supplemented with chloramphenicol and incubated for 7 days at 27°C. The remaining organs were fixed in a 10% formalin solution, and histological sections were prepared by conventional procedures. They were stained by H & E or PAS.Spores of E. exitialis ATCC 32890 and the mutant No. 1, and B. haptosporus MMC 68 and MMC 69 were recovered from the lungs of both groups of mice, while from other organs isolation was very rare.Spores of C. heterosporus ATCC 12941 were not recovered from any of the organs examined.Histopathologically, in the lungs of mice inoculated with E. exitialis ATCC 32890 and the mutant No. 1, a few zygospores were observed until the 21st day after inoculation. In general, these spores existed discretely in the alveolar tissues and their cellular reactions were very weak or entirely lacking. In the lungs of mice inoculated with C. heterosporus, a few conidia with or without cell infiltrates were observed in the sections 3 days after inoculation. In the lungs of mice inoculated with B. haptosporus MMC 68 and MMC 69, a few conidia and zygospores with or without cell infiltrates existed until the 14th day. Spores did not germinate during this experiment.These results indicate that the pathogenicity of these fungi is very feeble.
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