Tissue responses and fungus configurations. Histopathological and immunohistochemical studies of cutaneous sporotrichosis.
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概要
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The defence mechanism in sporotrichosis and the influence of corticosteroid (Cs) application were studied.<BR>A healthy adult man was intracutaneously injected 0.2ml Sporothrix schenckii spore suspension (1.8×106 spores) at 12 sites on his forearm. A half of the sites were applied Cs ointment. The injected sites were successively biopsied on day 3, 7, 14, 14, 21 and 28. The specimens were examined histopathologically and immunohistochemically using PAP method (antibodies used were; anti-MT-1, IgG, IgA, IgM, IgE, C3, lysozyme, α-antitrypsin and α1-antichymotrypsin, S-100 protein), The specimen taken on day 28 was also examined using ABC method (antibodies used were; anti-leu 1, 2a, 3a, 4, 6, 7, 14, M1 and anti-OKIA1).<BR>Histopathologically, control sites showed in its course abscess formation, transepidermal elimination of the abscess, maturation of Mo-Mφ around the abscess, and increase of the lymphocytes surrounding the granuloma. Fungal elements were seen mostly in the granuloma, but gradually decreased during the course. Cs inhibited these processes, and in Cs-applied group the number of fungal elements increased during the course to form a mass of innumerable spores seen in the specimen taken on day 28. Immunohistochemically, PMN, T cells (h/iT, s/cT), B cells, plasma cells (IgG-, IgA- and IgM-positive), histiocytes and giant cells were identified. IgE-, S-100 protein positive cells, NK cells, Langerhans cells, and C3-positive substances were not detected. The subpopulations in both groups were the same in essential.<BR>From these results it is assumed that PMN, Mφ T and B cells contribute and interact in the defence mechanism of sporotrichosis. It is also shown that Cs application facilitated the fungal proliferation. The inhibition of PMN aggregation may be a cause of this state.
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