A simple and reliable culturing method for production of arthrospores by dermatophytes.

概要

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Culturing conditions that are appropriate for production of large amounts of arthrospores by dermatophytes were determined. Namely, an inoculum of Trichophyton interdigitale NTM-105 spread on membrane filters (Millipore Co.) that were fixed on agar plate of each medium was incubated under various CO2 concentrations and temperatures. The media used were 1%, 2%, 3% and 4% peptone, Sabouraud, Sabouraud +3% NaCl, Sabouraud +6% NaCl, Sabouraud +12% NaCl and brain heart infusion agars. The developing surface growth was examined microscopically for efficiency of arthrospore formation (respective percentage of hyphal fragments with arthrospores, connected and disarticulated arthrospores, disarticulated arthrospores per 200 fragments), macro- and microconidia formation and wet growth weight. As a result, a new, simple culturing technique was devised as follows: The microconidial suspension of T. interdigitale was spread on a membrane filter fixed on a brain heart infusion agar plate and incubated at 30°C for 7 days in a jar containing 17% CO2. The culture resulted in good growth of T. interdigitale and the production of large amounts of its arthrospores. Furthermore, 50% or more of arthrospores were completely disarticulated, while neither macroconidia nor microconidia were seen. In this case, the colony surface of T. interdigitale was characterized by orange-colored, smooth, sticky growth and a lack of aerial hyphae. By scraping the membrane, a mass of arthrospores of T. interdigitale was easily collected for an experimental pathogenicity test. Examinations revealed similar effects in four of eight strains of T. mentagrophytes and two strains of T. interdigitale.
日本医真菌学会の論文

A simple and reliable culturing method for production of arthrospores by dermatophytes.

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概要

日本医真菌学会 | 論文

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