Histopathological study on serial changes in anastomosed carotid artery in rats; with special reference to intimal hyperplasia and re-endothelialization.:with special reference to intimal hyperplasia and re-endothelialization
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概要
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Repairing process following a microvascular end-to-end anastomosis of the common carotid artery was serially examined in 96 Wistar rats, weighted 250-350 gm, aged 14 weeks, using transmission electron microscopy (TEM) and immunohitochemical stain besides a light microscopic observation. We were paricularly interested in the process of intimal hyperplasia and re-endothelialization.<BR>Degeneration of the endothelium was observed shortly after anastomosis in the ischemic portion of the artery subjected to a temporary clipping. This was followed by its re-endothelialization, which proceeded for about 10 days.<BR>Intimal hyperplasia started on the 8-9th day mainly in the distal portion of the anastomosed area, and reached a maximum level at about the 14th day, followed by stabilizing thereafter.<BR>Morphological changes observed by TEM indicated that cells originally involved in intimal hyperplasia arose from the increased immature mesenchymal cells of the adventitia. They then migrated through cut portion of the tunica media. More superficial cells of the intimal hyperplasia differentiated into endothelial-like cells, while others differentiated into smooth muscle cells as also indicated by the results of immunohitochemical stain. Since human antibodies were used in this study, including laminin, collagen type IV, factor VIII, S-100 protein, muscleactin. UEA 1, a<SUB>1</SUB>-ACT and desmin, the stainability of the first five antibodies really supported this view.<BR>In conclusion, two types of the cells appeared to be involved in repair of the endothelium injured in microvascular anastomosis. They include : (1) cells arising from normal endothelium surrounding the anastomosed site, as is suggested conventionally, and (2) cells derived from endothelial-like cells originally involved in the intimal hyperplasia which arise from the increased immature mesenchymal cells of the adventitia.
- 一般社団法人 日本脳卒中学会の論文
一般社団法人 日本脳卒中学会 | 論文
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