RFLP Analysis of Japanese Quail's Genomic DNA Using Chicken's MHC Class I Probe.
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概要
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Restriction fragment length polymorphism (RFLP) analysis of Japanese quail (Coturnix japonica) was performed using probe (F10) of chicken's major histocompatibility complex (MHC) class I, and the relationship between its RFLP pattern by enhanced chemiluminescence (ECL) method and Ly antigen types in Ly system was examined. The results obtained are as follows; (1) The absorbance ratio (260nm/280nm) of extracted genomic DNA by using NaCl-Saponin solution for hemolysis of red blood cell was 1.866±0.034 and the concentration was 2.83±0.76mg/ml. Besides, genomic DNA of 3.78μg was extracted from 1μl volume of blood. (2) RFLP pattern of DNA digested with Pvu II in RWL strain (Ly1/Ly1), PNN strain (Ly2/Ly2) and SBPN strain (Ly3/Ly3) revealed 18-19 pieces of DNA bands, and a band of 8.3kb was observed in RFLP pattern of RWL strain. (3) The 8.3kb DNA band might be inherited from parents to offsprings, but it was not detected in the WEP strain (Ly1/Ly1). (4) ECL method as a labeling method of the probe was a valuable technique in RFLPanalysis.
- 日本家禽学会の論文
日本家禽学会 | 論文
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