IL 2 Activity and Dialysable Suppressive Factor in Chicken Lymphocyte Culture Medium.

概要

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Interleukin 2 (IL 2) activities estimated by lymphocyte activation were determined in the supernatants of phytohemagglutinin (PHA)-stimulated chicken peripheral blood lymphocyte (PBL) and splenocyte cultures. Dialysable T cell suppressive factor in culture supernatant of chicken PBL was also investigated. The peak activities of IL 2 in the supernatants of PHA-stimulated chicken lymphocyte cultures were observed during earlier period of incubation than those of mammalian species studied so far. The result suggests that chicken lymhocytes produce and secrete IL 2 more rapidly than mammalian lymphocytes, or chicken IL 2 is more labile to inactivate. Presence of dialysable (low molecular weight) factor that suppressed IL 2-promoted T cell activation in chicken PBL culture supernatant was suggested from the results that dialysis of PHA-stimulated PBL culture medium amplified the activation of Concanavalin A (ConA)-pretreated (IL 2 receptor positive) PBL, and that the dialysate of PHA-stimulated PBL culture medium conversely suppressed the activation of ConA-pretreated PBL. Accordingly, T cell activating factor (IL 2) and suppressive factor thought to be mixed in the mitogenstimulated chicken PBL culture supernatant. Secretion of the suppressive factor into culture medium may be a reason for lowering of IL 2 activity in the medium.
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