Efficient Expression System of Human Recombinant Laminin-5.
スポンサーリンク
概要
- 論文の詳細を見る
Laminin-5, a heterotrimer of laminin α3, β3, and γ2 chains, is an essential component of various epithelial basement membranes, and it strongly promotes cellular adhesion and motility in vitro. In this study, we established an efficient expression system of human recombinant laminin-5 (rLN5), in which full-length cDNAs encoding the human laminin α3, β3, and γ2 chains were introduced into the human embryonic kidney cell line HEK293. rLN5 was purified from the conditioned medium of the HEK293 transfectant (LN5-HEK) by immuno-affinity chromatography in a yield of 1 mg protein/liter, about 10 times higher than that of a natural LN5 from human gastric cancer cells. rLN5 was indistinguishable from the natural LN5 in its protein composition and biological activity. In addition, analysis of HEK293 transfectants expressing two exogenous LN5 subunits showed that the α3/γ2 chains and the β3/γ2 chains, but not the α3/β3 chains, were secreted as heterodimers, suggesting an important role of the γ2 chain in the association of the three LN5 subunits. The expression system of rLN5 can be used as an important tool to understand the biological functions of this laminin and may be applicable to future regenerative medicine.
- 社団法人 日本生化学会の論文
著者
-
Ogawa Takashi
Division of Cardiology, Fuji City Central Hospital
-
Miyazaki Kaoru
Division Of Cell Biology Kihara Insitutc For Biomedical Rescarch Yokohama City University.
-
TSUBOTA Yoshiaki
Division of Cell Biology, Kihara Institute for Biological Research and Graduate School of Integrated
-
Hirosaki Tomomi
Division Of Cell Biology Kihara Institute For Biological Research And Graduate School Of Integrated
-
Nakashima Yukiko
Division Of Cell Biology Kihara Institute For Biological Research And Graduate School Of Integrated
-
Ishida Kumiko
Division Of Cell Biology Kihara Institute For Biological Research And Graduate School Of Integrated
-
Kariya Yoshinobu
Division of Cell Biology, Kihara Institute for Biological Research and Graduate School of Integrated Sciences, Yokohama City University
関連論文
- Application of Air-solid Two-phase Flows to an Underground Digging System
- Numerical Investigation of a Self-Generating Spiral Flow
- High-Efficiency Soil Transportation with Spiral Airflow
- Gas-Solid Two-Phase Spiral Flow Investigation with PIV Techniques
- Deteriorative Effect of Smoking on Target Lesion Revascularization After Implantation of Coronary Stents With Diameter of 3.0 mm or Less
- Localization of ladsin, a homolog of Iaminin-5, in normal adult human tissues: immunohistochemical and immunoelectron microscopic studies
- Characterization of Matrix-degrading Proteinases and Their Inhibitors Secreted by Human Gynecological Carcinoma Cells
- IS-13 cDNA cloning of placentral protein 5 (PP5), a serine proteinase inhibitor, and its mRNA expression in human ovarian cancer cell lines
- Expression of Laminin-5 Enhances Tumorigenicity of Human Fibrosarcoma Cells in Nude Mice
- Purification and Identification of a Novel and Four Known Serine Proteinase Inhibitors Secreted by Human Glioblastoma Cells^1
- Role of Tissue Inhibitor of Metalloproteinases-2(TIMP-2)in Regulation of Pro-Gelatinase A Activation Catalyzed by Membrane-Type Matrix Metalloproteinase-1(MT1-MMP)in Human
- Characterization of Cell-Adhesive Proteins Secreted by Cell Lines Growing in Protein- and Lipid-Free Synthetic Medium: Mouse L・P3 Cells Secrete a Procollagen Molecule with Potent Cell-Attachment Activity
- Sole Expression of Laminin γ2 Chain in Invading Tumor Cells and Its Association with Stromal Fibrosis in Lung Adenocarcinomas
- Expression of Trypsin in Human Cancer Cell Lines and Cancer Tissues and Its Tight Binding to Soluble Form of Alzheimer Amyloid Precursor Protein in Culture
- Differential Expression of Laminin-k/Ladein Subunits in Human Tissues and Cancer Cell Lines and Teir Induction by Tumor Promoter and Growth Factors
- Regulation of Cell Adhesion and Type VII Collagen Binding by the β3 Chain Short Arm of Laminin-5 : Effect of Its Proteolytic Cleavage
- Efficient Expression System of Human Recombinant Laminin-5
- Matriptase activates stromelysin (MMP-3) and promotes tumor growth and angiogenesis
- Induction of MMP-9 expression in B16-BL6 melanoma cells by acidic extracellular pH is mediated by PLD-MAPKs and acidic sphingomyelinase pathways
- Anti-tumor Activity of Arginine Deiminase from Mycoplasma arginini and Its Growth-inhibitory Mechanism
- Inhibition of sea urchin fertilization by jaspisin, a specific inhibitor of matrix metalloendoproteinase
- Efficient Expression System of Human Recombinant Laminin-5.
- Role of Tissue Inhibitor of Metalloproteinases-2(TIMP-2) in Regulation of Pro-Gelatinase A Activation Catalyzed by Membrane-Type Matrix Metalloproteinase-1(MT1-MMP) in Human Cancer Cells.