Immunofluorescence for IgG and C3 in the swine kidney with trypsin-treated paraffin section: The influence of condition of formalin-fixation.
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In trypsin-treated paraffin sections of swine kidney samples fixed in 10% neutral buffered-formalin (NBF) for 6 to 11 months at room temperature and examined for IgG and C3 in the glomeruli by immunofluorescence, IgG was well preserved as in fresh frozen sections but C3 was remarkablly reduced. In samples fixed either in NBF or 10% formalin at 4°C or room temperature for one to 180 days, IgG was preserved better in NBF than formalin, while C3 fluorescence was apparently reduced during fixation, at room temperature.
- 公益社団法人 日本獣医学会の論文
公益社団法人 日本獣医学会 | 論文
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