Preparation of Photolabile and Cytocompatible Polymer Surface to Control Cell Adhesion and Detachment

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In this study cell attachment and detachment on the surface was selectively controlled using a photochemical reaction. We prepared a photolabile and cytocompatible polymer surface to control on cell attachment and detachment. As a polymer, poly [(2-methacryloyloxyethyl-phosphorylcholine(MPC)-co-n-butylmethacrylate(BMA)-co-photocleavable-monomer(PL)](PMB-PL) was synthesized. Photocleavable linker with active ester catches suitable for biomacromolecules, therefore cells bind for a culturing on a glass surface. The irradiation with UV light removes the cell adhesive molecules and biofouling surface is remained. Thus, it will be able to detach attached cells with photoirradiation on the surface. The photochemical activity of PMB-PL was examined by absorbance spectra. Features of before and after photoirradiated PMB-PL surface were characterized by Fourier transformed infrared reflection adsorption spectroscopy, X-ray photoelectron spectroscopy, and static contact angle measurement. Also, photo-induced detachment of cells on the surface was examined. As a result, 91% of seeded 4×104 cells were attached onto PMB-PL surface and 67% of attached cells were detached by photochemical reaction. We expect expansion of this tool can be applied for various field, e.g., single cell analysis system, cell and tissue engineering.
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