Controlling cell functions by encapsulation with cytocompatible phospholipid polymer hydrogel
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概要
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To control functions of cells during their culture period in vitro is important for providing cells in the cell engineering fields. We designed a novel soft biodevice for realizing cell engineering. That is, spontaneously forming and highly cytocompatible hydrogel system composed of poly (2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid) (PMBV) and poly (vinyl alcohol) (PVA), was prepared to encapsulate cells as "Cell Container". This hydrogel was reversibly dissociated by addition of sugar derivatives. When mouse fibroblast (L929) cell lines and human hepatocellular liver carcinoma (HepG2) cell lines were encapsulated in the PMBV/PVA hydrogel, the cells could proliferate slowly compared with those on conventional tissue culture plate and form cell clusters derived by expansion of the single cells, without forming aggregations. After the dissociation of the PMBV/PVA hydrogel, the distribution of cell cycle phase of L929 cells was analyzed. It was found that the PMBV/PVA hydrogel lead a synchronization of cell cycle phase of L929 cells to G0/G1 phase. As other cell-specific function of HepG2, albumin production and releasing from HepG2 was evaluated. The PMBV/PVA hydrogel did not induce a significant reduction of albumin production from HepG2. The PMBV/PVA hydrogel could preserve cells for long term inhibiting the excessive proliferation, provide normalized cells with uniformly cell cycle phase, and maintain the cell-specific function. Thus, we concluded that the PMBV/PVA hydrogel will be a novel and powerful device to control cell functions in cell engineering fields.
- 一般社団法人 日本MRSの論文
一般社団法人 日本MRS | 論文
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