Glycoprotein recognition for cell capturing on artificial lectin surface

概要

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We prepared a new biointerface for obtaining specific recognition of glycoproteins having polysaccharide chains to capture corresponding cells by a phospholipid polymer having phenylboronic acid moiety (PMBV), so-called artificial lectin. The PMBV was coated on the surfaces of Au substrate and tissue culture polystyrene. The affinity of the PMBV toward the glycoproteins and its effects on cell adhesion were evaluated. X-ray photoelectron spectroscopic analysis revealed that the PMBV existed on both substrates. Although adsorption of protein without polysaccharide chain was suppressed, glycoprotein as fibronectin (FN) adsorbed effectively, which was confirmed by surface plasmon resonance. Kinetic parameters calculated by SPR measurements showed high affinity between PMBV and FN, 1.4×105 (M-1). Cell adhesion and growth behavior on PMBV was observed using L-929 fibroblast cells. It was demonstrated that cells proliferated well on PMBV surface and maintained their sphere morphology. Also, the activity of cells was kept in higher level on the PMBV surface compared with conventional surface.
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