Assessment of the Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Mycoplasma pneumoniae in Pediatric Community-Acquired Pneumonia
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概要
- 論文の詳細を見る
Rapid diagnosis of Mycoplasma pneumoniae pneumonia is required for timely treatment with effective antibiotics; however, PCR-based methods are often too expensive and technologically intensive for general use in clinical practice. In this study, the efficacy of the loop-mediated isothermal amplification (LAMP) assay for diagnosis of M. pneumoniae pneumonia in clinical practice was prospectively evaluated. From July 2011 to March 2012, 531 children hospitalized for community-acquired pneumonia were enrolled. In all patients, throat swabs were obtained on admission for the detection of M. pneumoniae DNA, and paired serum samples were obtained to assay M. pneumoniae particle agglutination (PA) antibody titers. M. pneumoniae pneumonia was diagnosed by either a positive LAMP assay or an increase of 4-fold or greater in the PA titer. Overall, 271 children (51.0% of the patients with pneumonia) were diagnosed with M. pneumoniae pneumonia. Among these, 258 (95.2%) and 248 (91.5%) were identified by the LAMP assay and serological tests, respectively. When the results of serological tests were considered as standard, the sensitivity, specificity, and positive and negative predictive values of the LAMP assay were 94.8%, 91.9%, and 91.1% and 95.2%, respectively. The median duration of pharyngeal carriage, as measured by the LAMP assay, was 9.5 days. Thus, the LAMP assay is useful in the rapid diagnosis of M. pneumoniae pneumonia.
- National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committeeの論文
著者
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Ozaki Takao
Department Of Paediatrics Showa Hospital
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Takemoto Koji
Department Of Biotechnology Faculty Of Engineering Okayama University
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Iwata Yasushi
Department Of Applied Chemistry Nagoya Institute Of Technology
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Nishimura Naoko
Department Of Applied Biological Science Tokyo Noko University
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NAKANE Kazumasa
Department of Clinical Laboratory, Konan Kosei Hospital
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HOSONO Haruki
Department of Pediatrics, Konan Kosei Hospital
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GOTOH Kensei
Department of Pediatrics, Konan Kosei Hospital
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OHSHIMA Yasunori
Department of Pediatrics, Konan Kosei Hospital
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FUNAHASHI Keiji
Department of Clinical Laboratory, Konan Kosei Hospital
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Takeuchi Suguru
Department of Pediatrics, Konan Kosei Hospital
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Hattori Fumihiko
Department of Pediatrics, Konan Kosei Hospital
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Horiba Kazuhiro
Department of Pediatrics, Konan Kosei Hospital
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Isaji Mai
Department of Pediatrics, Konan Kosei Hospital
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Okai Yu
Department of Pediatrics, Konan Kosei Hospital
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Takemoto Koji
Department of Pediatrics, Konan Kosei Hospital
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