BMP-2 による頰脂肪体由来成体幹細胞の骨形成能

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概要

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• Bone morphogenetic proteins (BMPs) in combination with stem cells gain more significance for their use in bone tissue engineering. The mesenchymal stem cell can be differentiated into osteoblast by the treatment of BMP. The aim of this study is to characterize the osteogenic differentiation process of adult stem cells derived from buccal fat pad according to BMP-2 within culture media and decide the appropriate concentration of BMP-2 to facilitate osteogenesis.The authors procured the stem cell from buccal fat pad and analyzed for presence of stem cell by flow cytomety against CD-34, CD-105 and STRO-1. The buccal fat derived stem cells (BFDC) were treated by application of the different concentration with BMP-2 of 0, 10, 50, 100 and 200 ng/ml, respectively. And their ability to differentiate into osteogenic pathway were checked by alkaline phosphatase (ALP) staining, Alizarin red staining and RT-PCR for osteocalcin (OC) gene expression at 7, 14 and 21day of culture. Flow cytometric analysis and biochemical assays demonstrated that BFDC might be a distinguished stem cells, and mineralization was accompanied in proportion to BMP-2 concentration. However, with 100ng/ml concentration of BMP-2, the BFDC demonstrated most efficent staining pattern of ALP and Alizarin red. The feasibilty of the osteogenic differentiation in the group of both 50 ng/ml and 200 ng/ml of BMP-2 showed similar activity and relatively weaker than that of 100 ng/ml.These results suggest that the BMP-2 stimulate osteogenesis by BFDC effectively and that bone induction might be controlled through negative regulatory feedback in higher concentration.

• Japanese Society of Oral and Maxillofacial Surgeonsの論文

著者

• 李 溢奎

  カトリック大学校大学院統合医学科

• 表 聖雲

  カトリック大学校医科大学歯科学教室口腔顎顔面外科

• 朴 哲憲

  カトリック大学校臨床歯科学大学院インプラント学科

• 金 昌鉉

  カトリック大学校医科大学歯科学教室口腔顎顔面外科

関連論文

• BMP-2による頬脂肪体由来成体幹細胞の骨形成能
• BMP-2 による頰脂肪体由来成体幹細胞の骨形成能

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