Steely Enzymes Are Involved in Prestalk and Prespore Pattern Formation

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概要

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• 4-Methyl-5-pentylbenzene-1,3-diol (MPBD), a product of SteelyA enzyme, controls Dictyostelium spore maturation. Since the expression of stlA split the in early and terminal stages, we cannot exclude the possibility that MPBD regulates spore differentiation from the early stage by creating a bias between the cells. 1-(3,5-Dichloro-2,6-dihydroxy-4-methoxyphenyl) hexan-1-on (DIF-1), a product of SteelyB, was identified as the major stalk cell inducer by in vitro assay, but in vivo assay revealed that DIF-1 induces only prestalkB (pstB) and prestalkO (pstO) cells and, that the major prestalkA (pstA) cells differentiated without DIF-1. In order to determine mechanism of polyketide regulated pattern formation, we examined the spatial expression patterns of prestalk and prespore markers in stlA and stlB knockout mutants. We found that MPBD regulates spore maturation at the culmination stage. We also found that the stlA and stlB double-knockout mutant lost pstA marker gene expression.

著者

• Fukuzawa Masashi

  Department Of Biology Faculty Of Agriculture And Life Science Hirosaki University

• SATO Yukie

  Graduate School of Science and Technology, Sophia University

• KAGAMI Haruna

  Department of Materials and Life Sciences, Sophia University

• NARITA Takaaki

  Graduate School of Science and Technology, Sophia University

• SAITO Tamao

  Department of Materials and Life Sciences, Sophia University

関連論文

• Differentiation inducing factors in Dictyostelium discoideum : A novel low molecular factor functions at an early stage (s) of differentiation
• Prespore cell inducing factor, ψ factor, controls both prestalk and prespore gene expression in Dictyostelium development
• Control of prestalk-cell differentiation by transcription factors
• Steely Enzymes Are Involved in Prestalk and Prespore Pattern Formation

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