Dynamic Changes in the Initial Colonization of Actinomyces naeslundii and Streptococcus gordonii Using a New Animal Model

スポンサーリンク

概要

• 論文の詳細を見る

• Actinomyces naeslundii and Streptococcus gordonii are the predominant bacteria and initial colonizers of oral microflora. The binding of A. naeslundii and S. gordonii and the interaction between them on the salivary pellicle-coated tooth surface play an important role in the biofilm development. Recently, we reported that NOD/SCID.e2f1− mice are a useful model for studying oral biofilm formation by Streptococcus mutans on the tooth surface. In this study, we aimed to determine whether NOD/SCID.e2f1− mice can be used for studying oral colonization of A. naeslundii and S. gordonii. Colonization of A. naeslundii in mice fed with 1% sucrose water for 24 h before inoculation was higher than that among mice fed with sucrose water for 1 h. A. naeslundii colonization using mixed species-inoculation was lower than that using single-species inoculation 30–90 min after inoculation; however, the colonization was higher 120–180 min after inoculation. The mixed inoculation induced better colonization of S. gordonii than single-species inoculation 60–180 min after inoculation. Polyclonal and fluorescein isothiocyanate-labeled antibody stained bacteria showed better colonization of S. gordonii when a mixed culture is used in vivo. NOD/SCID.e2f1− mice were useful for studying the initial colonization of A. naeslundii and S. gordonii. Long-term supply of sucrose water creates a favorable environment for the initial colonization of A. naeslundii that, in turn, supports the colonization of S. gordonii.

著者

• Senpuku Hidenobu

  Department of Bacteriology I, National Institute of Infectious Diseases

• Zhang Xi

  Stomatological Hospital of Tianjin Medical University

関連論文

• Survey of dental hygienists and healthcare workers for microorganisms in the oral cavity
• Model Mouse Designed for Oral Biofilm Formation Studies
• Establishnent of model animal for oral infection using nonobese diabetic (NOD) mice.
• Effects of Enterococcus faecium on Streptococcus mutans Biofilm Formation Using Flow Cell System
• Hinokitiol Inhibits Candida albicans Adherence to Oral Epithelial Cells
• Impact of routine oral care on opportunistic pathogens in the institutionalized elderly
• Comparison of autoaggregation and surface hydrophobocity between Porphyromonas gingivalis 33277 strain and the isogenic mutants, hagB/C and galE strains
• Dynamic Changes in the Initial Colonization of Actinomyces naeslundii and Streptococcus gordonii Using a New Animal Model

スポンサーリンク