Vitamin E Deficiency Induces Axonal Degeneration in Mouse Hippocampal Neurons

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概要

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• Several lines of evidence demonstrate the relationship between vitamin E deficiency and cognitive dysfunction in rodent models, but little is known about the underlying mechanisms. In this study, we found axonal injury in the hippocampal CA1 region of vitamin E-deficient and normal old mice using immunohistochemical assay. The number of cells in the hippocampal CA1 region of vitamin E-deficient mice and normal old mice was significantly lower than in normal young mice. It is well known that collapsin response mediator protein (CRMP)-2 plays a crucial role in the maintenance of axonal conditions. The expressions of CRMP-2 in the cerebral cortex and hippocampus of vitamin E-deficient mice were significantly lower than both the regions of normal ones. In normal old mice, the expression of CRMP-2 in the cerebral cortex was significantly lower than in the normal ones. In addition, the appearance of microtubule-associated protein (MAP)-light chain 3 (LC3), a major index of autophagy, was higher in the cerebral cortex and hippocampus of vitamin E-deficient mice than in normal young and old mice. These results indicate that axonal degeneration is induced in living tissues, but not cultured cells, and that changes in CRMP-2 and MAP-LC3 may underlie vitamin E-deficiency-related axonal degeneration.

著者

• Koike Tatsuro

  Molecular Neurobiology Laboratory, Division of Biological Sciences, Graduate School of Science, Hokk

• FUKUI Koji

  Physiological Chemistry Laboratory, Department of Bioscience and Engineering, College of Systems Engineering and Sciences, Shibaura Institute of Technology

• KAWAKAMI Hiroaki

  Physiological Chemistry Laboratory, Department of Bioscience and Engineering, College of Systems Engineering and Sciences, Shibaura Institute of Technology

• HONJO Tatsuki

  Physiological Chemistry Laboratory, Department of Bioscience and Engineering, College of Systems Engineering and Sciences, Shibaura Institute of Technology

• OGASAWARA Reiko

  Physiological Chemistry Laboratory, Department of Bioscience and Engineering, College of Systems Engineering and Sciences, Shibaura Institute of Technology

• TAKATSU Hirokatsu

  Life Support Technology Research Center, Research Organization for Advanced Engineering, Shibaura Institute of Technology

• SHINKAI Tadashi

  Homeostatic Regulation Laboratory, Department of Bioscience and Engineering, College of Systems Engineering and Sciences, Shibaura Institute of Technology

• URANO Shiro

  Biochemistry Laboratory, Department of Bioscience and Engineering, College of Systems Engineering and Sciences, Shibaura Institute of Technology

• KOIKE Tatsuro

  Molecular Neurobiology Laboratory, Graduate School of Life Science, Hokkaido University

関連論文

• Microglial Response Factor (MRF) -1 : Constitutive Expression in Ramified Microglia and Upregulation upon Neuronal Death Induced by Ischemia or Glutamate Exposure(Neurobiology)
• Vitamin E Deficiency Induces Axonal Degeneration in Mouse Hippocampal Neurons

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