Feasibility of Histological Scoring and Colony Count for Evaluating Infective Severity in Mouse Vaginal Candidiasis
スポンサーリンク
概要
- 論文の詳細を見る
Qualitative measurement of the infective level is relatively difficult in experimental vaginal candidiasis. Female BALB/c mice aged 8 to 10 weeks were randomly divided into E1, E2 and E0 groups, which received subcutaneous injection of 0.05 mg, 0.1 mg of estradiol benzoate or 0.1 ml soybean oil 3 days before vaginal inoculation, respectively, and hormone treatment continued every other day thereafter. Each group was further divided into infected and noninfected subgroups. The infected mice were inoculated intravaginally with 10 μl (5 × 104 conidia) of Candida albicans suspension, while the noninfected mice were inoculated with 10 μl phosphate-buffered saline. Direct microscopic examination, colony count and vaginal histopathology including infection degree and inflammation extent were performed at 3, 7 and 14 days post inoculation. Estrogen treatment increased the vaginal fungal burden and extent of infection and inflammation compared with the control group, and 0.3 mg/week estrogen generally induced more severe infection and inflammation than 0.15 mg/week estrogen did. Colony count peaked on day 3 and decreased remarkably after 7 days. Infection score increased gradually during the first 7 days and decreased on day 14, while inflammation extent exacerbated progressively over the course of 14 days. This study demonstrates that the modified histological scoring system might be more feasible than colony count for evaluation of infectivity and dynamic change in experimental vaginal candidiasis.
著者
-
Luo Dan
Department Of Dermatology The First Affiliated Hospital Of Nanjing Medical University
-
Zhou Ying
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Fan Yi-Ming
Department of Dermatology, Affiliated Hospital of Guangdong Medical College, No.57, Renmin Avenue, Xiashan District, Zhanjiang, Guangdong, 524001, P. R. China
-
ZHANG Jin-E
Department of Dermatology, Affiliated Hospital of Guangdong Medical College, No.57, Renmin Avenue, Xiashan District, Zhanjiang, Guangdong, 524001, P. R. China
-
CHEN Rong-Yi
Department of Dermatology, Affiliated Hospital of Guangdong Medical College, No.57, Renmin Avenue, Xiashan District, Zhanjiang, Guangdong, 524001, P. R. China
-
Yang Yan-Ping
Department of Dermatology, Affiliated Hospital of Guangdong Medical College, No.57, Renmin Avenue, Xiashan District, Zhanjiang, Guangdong, 524001, P. R. China
-
Zhou Ying
Department of Dermatology, Affiliated Hospital of Guangdong Medical College, No.57, Renmin Avenue, Xiashan District, Zhanjiang, Guangdong, 524001, P. R. China
-
Luo Dan
Department of Dermatology, Affiliated Hospital of Guangdong Medical College, No.57, Renmin Avenue, Xiashan District, Zhanjiang, Guangdong, 524001, P. R. China
関連論文
- Enhancement of recombinant enzyme activity in cpxA-deficient mutant of Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- A new arginine substitution mutation of DSRAD gene in a Chinese family with dyschromatosis symmetrica hereditaria
- Ginsenoside Rb1 Suppresses Ultraviolet Radiation-Induced Apoptosis by Inducing DNA Repair
- Generalized Relation Theory of Torque Balance Method for Azimuthal Anchoring Measurements
- A Two-Dimensional Stokes Parameter Method for Determination of Cell Thickness and Twist Angle Distributions in Twisted Nematic Liquid Crystal Devices
- A Novel Method for Determining the Cell Thickness and Twist Angle of a Twisted Nematic Cell by Stokes Parameter Measurement
- Acute stress enhances contact dermatitis by promoting nuclear factor-κB DNA-binding activity and interleukin-18 expression in mice
- Electrooptical and Response/Relaxation Properties of Liquid Crystal Cells in In-plane Switching Mode with Polyvinylcinnamate Photoinduced Alignment Layer
- Effect of Azithromycin on Patients with Diffuse Panbronchiolitis: Retrospective Study of 51 Cases
- Safety and Pharmacokinetic Studies of Silodosin, a New α1A-Adrenoceptor Selective Antagonist, in Healthy Chinese Male Subjects
- Surgical management to conjoined twins in Shanghai area
- 20-P2-CJK12 Therapy of Dyslipidemia in Patients with Type-2 Diabetes
- 20-P2-CJK11 Lipoatrophy with insulin therapy
- Determination of silodosin in human plasma by liquid chromatography-tandem mass spectrometry
- Study on Ward Planning of Acute Hospitals from the Nurses' Standpoint(Architectural/Urban Planning and Design)
- Dynamic Young's Modulus and Mechanical Properties of Ti-Hf Alloys
- Dental Work Force in China
- Restoration of antibiotic susceptibility in fluoroquinolone-resistant Escherichia coli by targeting acrB with antisense phosphorothioate oligonucleotide encapsulated in novel anion liposome
- An Improved Stokes Parameter Method for Determination of the Cell Thickness and Twist Angle of Twisted Nematic Liquid Crystal Cells
- Feasibility of Histological Scoring and Colony Count for Evaluating Infective Severity in Mouse Vaginal Candidiasis
- IN-24 Pharmacists' Role in the Management of Chronic Disease(International Poster)
- A Method for Determining Elastic Constants of Nematic Liquid Crystals at High Electric Fields