細胞を用いた多チャンネル化学量センサのための電極一体型細胞アレイ化流路の作製

概要

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This paper shows the fabrication of a fluidic device for measuring of membrane potential changes of multiple cells. The device is composed of poly(methyl methacrylate) (PMMA) and a silicon substrate that has needle-shaped structures for electrodes. The electrodes are for "two-electrode voltage clamping method" that can measure ion flows across cell membrane using an amplifier and two electrodes inserted into a cell. In this device, Xenopus laevis oocytes can be arrayed automatically in the fluidic channel and penetrated by electrode structures at the same time. To construct the fluidic device integrated with electrodes, we simulated and calculated the fluid behavior for oocytes array and investigated the flow rate for electrodes insertion into oocytes.