Xanthoceraside Inhibits Pro-inflammatory Cytokine Expression in Aβ25–35/IFN-γ–Stimulated Microglia Through the TLR2 Receptor, MyD88, Nuclear Factor-κB, and Mitogen-Activated Protein Kinase Signaling Pathways

スポンサーリンク

概要

• 論文の詳細を見る

• An accumulating body of evidence suggests that Alzheimer's disease (AD) is associated with microglia-mediated neuroinflammation and pro-inflammatory cytokine expression. Therefore, the suppression of neuroinflammation and pro-inflammatory cytokine might theoretically slow down the progression of AD. Xanthoceraside, a novel triterpenoid saponin extracted from the husks of Xanthoceras sorbifolia B<span style="font-variant: small-caps;">unge</span>, has potent antiinflammatory and neuroprotective effects. However, the molecular mechanism underlying its anti-inflammatory action remains unclear. In the present study, we attempted to determine the effects of xanthoceraside on the production of pro-inflammatory mediators in amyloid β25–35 (Aβ25–35) / interferon-γ (IFN-γ)-stimulated microglia. Our results indicated that xanthoceraside (0.01 and 0.1 μM) significantly inhibited the release of nitric oxide (NO) and pro-inflammatory cytokines interleukin-1β and tumor necrosis factor-α in a concentration-dependent manner. Reverse transcriptase–polymerase chain reaction and western blotting analyses showed that xanthoceraside decreased the Aβ25–35/IFN-γ–induced production of cyclooxygenase-2 and inducible NO synthase. These effects were accompanied by inhibited activities of nuclear factor-κB and mitogen-activated protein kinase through Toll-like receptor 2 in a myeloid differentiation protein 88–dependent manner. Our results provide support for the therapeutic potential of xanthoceraside in AD.

著者

• Zou Li-bo

  Department Of Life Science And Biopharmaceutics Shenyang Pharmaceutical University

• Chi Tian-yan

  Department Of Life Science And Biopharmaceutics Shenyang Pharmaceutical University

• Ji Xue-fei

  Department Of Life Science And Biopharmaceutics Shenyang Pharmaceutical University

• Qi Yue

  Department Of Epidemiology Beijing An Zhen Hospital Capital Medical University Beijing Institute Of Heart Lung And Blood Vessel Diseases

• Zou Li-Bo

  Department of Pharmacology, Life Science and Biopharmaceutics School, Shenyang Pharmaceutical University, China

• Wang Li-Hua

  Shenyang Institute of Applied Ecology, Chinese Academy of Sciences, China

• Jin Ge

  Department of Pharmacology, Life Science and Biopharmaceutics School, Shenyang Pharmaceutical University, China

• Pan Jin-Jin

  Department of Pharmacology, Life Science and Biopharmaceutics School, Shenyang Pharmaceutical University, China

• Chi Tian-Yan

  Department of Pharmacology, Life Science and Biopharmaceutics School, Shenyang Pharmaceutical University, China

• Ji Xue-Fei

  Department of Pharmacology, Life Science and Biopharmaceutics School, Shenyang Pharmaceutical University, China

関連論文

• The relationship between apolipoprotein E ε2/ε3/ε4 polymorphisms and hypertension : a meta-analysis of six studies comprising 1812 cases and 1762 controls
• Protective Effect of Daidzein against Impairment of Learning and Memory Induced by Cerebral Ischemia-reperfusion in Mice
• Association of the angiotensin II type I receptor gene +1166 A>C polymorphism with hypertension risk : evidence from a meta-analysis of 16474 subjects
• Angiotensin converting enzyme D allele is associated with an increased risk of type 2 diabetes : evidence from a meta-analysis
• Association of an apolipoprotein E polymorphism with circulating cholesterols and hypertension : a meta-based Mendelian randomization analysis
• Xanthoceraside Inhibits Pro-inflammatory Cytokine Expression in Aβ25–35/IFN-γ–Stimulated Microglia Through the TLR2 Receptor, MyD88, Nuclear Factor-κB, and Mitogen-Activated Protein Kinase Signaling Pathways

スポンサーリンク