Amikacin-induced Fin Reduction is Mediated by Autophagy

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概要

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• Despite its medical use, little is known about the mechanisms underlying amikacin-induced embryotoxicity, including fin reduction, in zebrafish. In this study, we examined the expression of well-known autophagy markers mTOR (target of rapamycin), atg10 (autophagy-related gene), atg12 and LC3 (mammalian homolog of Atg8) in amikacin-treated zebrafish embryos. Our results indicated that the mRNA expression level of atg12 in the amikacin-treated group was significantly increased by 1.5-fold (p<0.05) compared with the corresponding mock control group, while the expression levels of atg10 and mTOR were significantly decreased by 0.74-fold (p<0.05) and 0.58-fold (p<0.05), respectively. Western blot analysis revealed that LC3 protein expression was induced by amikacin. Taken together, these data suggest that amikacin-induced fin reduction is mediated by fin cell autophagy.

著者

• Chen Yau-Hung

  Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan, ROC

• WANG Yun-Hsin

  Division of Basic Research, Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan

• Tsai I-Ting

  Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan, ROC

• Chen Ying-Hsin

  Department of Emergency Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan

関連論文

• Amikacin-induced Fin Reduction is Mediated by Autophagy
• Overdose of D-serine Induces Movement Disorder and Neuromuscular Changes of Zebrafish Larvae

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