Detection of Bovine Group A Rotavirus Using Rapid Antigen Detection Kits, RT-PCR and Next-Generation DNA Sequencing
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概要
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We investigated the sensitivity of human rotavirus rapid antigen detection (RAD) kits, RT-PCR and next-generation DNA sequencing (NGS) for detection of bovine group A rotavirus (RVA). The Dipstick 'Eiken' Rota (Dipstick) showed the highest sensitivity out of the seven RAD kits against all selected strains in limited dilution analyses, which was consistent with the results of equine rotavirus previously reported. RT-PCR had 100-103-fold higher sensitivity than the Dipstick. The NGS using RT-PCR negative thirteen fecal samples revealed that all samples yielded RVA reads and especially two of them covered all 11 genome segments. Moreover, mapping reads to reference sequences allowed genotyping. The NGS would be sensitive and useful for analysis of less dependent on specific primers and screening presumption of genotype.
著者
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NAGAI Makoto
Research Center for the Early Universe and Department of Physics, The University of Tokyo
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Fujii Yoshiki
Department Of Virology 1 National Institute Of Infectious Diseases
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Katayama Kazuhiko
Department Of Epidemiology Infectious Disease Control And Prevention Hiroshima University
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Tsunemitsu Hiroshi
National Agriculture And Food Res. Organization Ibaraki
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Murakami Toshiaki
Ishikawa Prefectural Livestock Research Center
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TAKAI Hikaru
Ishikawa Nanbu Livestock Hygiene Service Center, Saida, Kanazawa, Ishikawa 920–3101, Japan
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MINAMI-FUKUDA Fujiko
Ishikawa Nanbu Livestock Hygiene Service Center, Saida, Kanazawa, Ishikawa 920–3101, Japan
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OZAWA Tadashi
Ishikawa Nanbu Livestock Hygiene Service Center, Saida, Kanazawa, Ishikawa 920–3101, Japan
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TSUCHIAKA Shinobu
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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OKAZAKI Sachiko
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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KATAYAMA Yukie
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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OBA Mami
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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NISHIURA Naomi
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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SASSA Yukiko
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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OMATSU Tsutomu
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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FURUYA Tetsuya
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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KOYAMA Satoshi
Laboratory of Ethology, Department of Veterinary Medicine, Faculty and Agriculture, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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SHIRAI Junsuke
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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MIZUTANI Tetsuya
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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KATAYAMA Kazuhiko
Department of Virology II, National Institute of Infectious Diseases, Gakuen, Musashi-murayama, Tokyo 208–0011, Japan
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NAGAI Makoto
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183–8509, Japan
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MURAKAMI Toshiaki
Ishikawa Nanbu Livestock Hygiene Service Center, Saida, Kanazawa, Ishikawa 920–3101, Japan
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