Molecular Analysis of Parapoxvirus Detected in Eight Calves in Japan

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概要

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• Molecular analysis of parapoxvirus envelope genes was performed. Parapoxvirus DNA was detected in eight calves from eight farms in Iwate Prefecture, Japan, between April and September 2010. Seven of the detected viruses were identified as bovine papular stomatitis virus (BPSV) by sequencing, because their nucleotide identity was more than 96.8% similar compared with BPSV strain V660. Among them, two formed a subgroup, because their amplicons were digested with Xmn I (a marker for BPSV) and Hinc II, and exhibited a T61C nucleotide substitution in the sequenced region. The remaining virus was pseudocowpox virus that had not been reported previously in Japan. Our results demonstrate the presence of a new BPSV variant in Japan, with genetic variability in the envelope gene.

著者

• YAEGASHI Gakuji

  Iwate Prefecture Central Livestock Hygiene Service Center

• YAEGASHI Gakuji

  Iwate Prefecture Central Livestock Hygiene Service Center, 390–5 Sunagome, Takizawa-mura, Iwate 020–0173, Japan

• CHIBA Shin

  Iwate Prefecture Central Livestock Hygiene Service Center, 390–5 Sunagome, Takizawa-mura, Iwate 020–0173, Japan

• MURAKAMI Kenji

  Food Animal Medicine and Food Safety Research Center (FAMS), Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550, Japan

• SASAKI Ieharu

  Iwate Prefecture Central Livestock Hygiene Service Center, 390–5 Sunagome, Takizawa-mura, Iwate 020–0173, Japan

関連論文

• Molecular Analysis of Parapoxvirus Detected in Eight Calves in Japan
• Occurrence of Equine Coital Exanthema in Pastured Draft Horses and Isolation of Equine Herpesvirus 3 from Progenital Lesions

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