Subcutaneous or Oral Immunization of Mice with Lactococcus lactis Expressing F4 Fimbrial Adhesin

概要

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Enterotoxigenic Escherichia coli (ETEC) is one of the most common causes of diarrhea in neonatal and post-weaning piglets. Fimbrial adhesion of ETEC has been considered an important colonization factor with antigenicity. To safely and effectively deliver the F4 (K88) fimbrial adhesin FaeG to the immune system, we have previously constructed the secretory expression vector of pNZ8112-faeG and FaeG was produced in cytoplasmic form in Lactococcus lactis. In this work, BALB/c mice were immunized with recombinant L. lactis to further determine the immunogenicity of recombinant FaeG (rFaeG) via the subcutaneous or oral route. Subcutaneous immunization in mice with recombinant L. lactis induced a significant increase in the F4-specific serum IgG titer and the number of antibody-secreting cells (ASC) in the spleen. Oral immunization of mice with recombinant L. lactis induced mucosal and systemic F4-specific immune responses, and increased the number of ASC in the spleen, mesenteric lymph nodes, and Peyer's patches. High-dose (2.8 × 1011 CFU) recombinant strains and adjuvant cholera toxin B subunit enhanced specific mucosal immune responses. The results suggest the feasibility of delivering rFaeG expressed in L. lactis to the immune system in order to induce F4-specific immune response.