Erythropoietin Prevents Hypoxia-Induced GATA-4 Ubiquitination via Phosphorylation of Serine 105 of GATA-4

スポンサーリンク

概要

• 論文の詳細を見る

• Erythropoietin (EPO), an essential hormone for erythropoiesis, can provide protection against myocardial ischemia/reperfusion (I/R) injury and hypoxic apoptosis. GATA-4 is a zinc finger transcription factor, and its activation and post-translational modification are essential components in the transcriptional response to hypoxia. GATA-4 has also been reported to play a role in the cellular mechanisms of EPO-induced myocardial protection against I/R injury. In this study, we aimed to investigate the influence of EPO on GATA-4 protein stability and post-translational modification under hypoxic conditions without reperfusion. EPO induced cell viability under long-term hypoxia. EPO significantly increased phosphorylation of GATA-4 via the extracellular signal-regulated kinase (ERK) signaling pathway and reduced hypoxia-induced GATA-4 ubiquitination, which enhanced GATA-4 stability under hypoxia. ERK activation by over-expression of constitutively active mitogen-activated protein kinase 1 (MEK1) strongly increased GATA-4 phosphorylation and its protein levels and decreased GATA-4 ubiquitination under hypoxia. Despite ERK activation, GATA-4 ubiquitination was not affected under hypoxia in a GATA-4-S105A mutant. Under hypoxic condition without reperfusion, EPO-induced ERK activation was associated with post-translational modification of GATA-4, mediated by enhancement of phosphorylation of GATA-4 at Ser-105. Subsequent attenuation of GATA-4 ubiquitination led to increases in GATA-4 protein stability, which resulted in increased cell viability under hypoxia.

著者

• Kim Si

  Department of Anesthesiology and Pain Medicine, College of Medicine, Kyungpook National University Hospital

• Jun Ji

  Severance Biomedical Science Institute, Yonsei University College of Medicine

• Shin Eun

  Anesthesia and Pain Research Institute, Yonsei University Health System

• Kim Ji

  Anesthesia and Pain Research Institute, Yonsei University Health System

• Shim Jae-Kwang

  Anesthesia and Pain Research Institute, Yonsei University Health System

• Kwak Young-Lan

  Severance Biomedical Science Institute, Yonsei University College of Medicine

関連論文

• Diffusion Tensor MRI and Fiber Tractography of Cerebellar Atrophy in Phenytoin Users
• II C15 Application of diffusion tensor imaging in the evaluation of cerebellar atrophy associated with phenytoin
• Cell Cycle Inhibitory Activity of 4-Hydroxy-3-(3'-methyl-2'-butenyl)-benzoic Acid from Curvularia sp. KF119
• Bilateral Cytomegalovirus Retinitis with Unilateral Optic Neuritis in Good Syndrome
• Conjunctival Nevus-like Lesions Originating from a Sclerotomy Site After 23-Gauge Transconjunctival Sutureless Vitrectomy
• Effects of Temperature and Hydraulic Retention Time on Anaerobic Digestion of Food Waste(BREWING AND FOOD TECHNOLOGY)
• Purification and Characterization of Superoxide Dismutase from Aerobacter aerogenes(Microbiology & Fermentation Industry)
• Cytopathology of Cervical Cancer in Korea
• Erythropoietin Prevents Hypoxia-Induced GATA-4 Ubiquitination via Phosphorylation of Serine 105 of GATA-4

スポンサーリンク