Construction of an Engineering Strain Producing High Yields of α-Transglucosidase via Agrobacterium tumefaciens-Mediated Transformation of Asperillus niger
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概要
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In this study, Agrobacterium tumefaciens-mediated transformation (ATMT) was used in breeding industrial strains for the purpose of improving α-transglucosidase production. Firstly, an efficient ATMT system for Asperillus niger was established by optimization of several influencing factors, in which transformation efficiency was improved up to 14-fold compared with the initial conditions. Furthermore, binary vector pBI-Glu containing an α-transglucosidase expression cassette was constructed and transferred into Agrobacterium tumefaciens LBA4404 in order to infect A. niger. By the efficient ATMT method, the gene for α-transglucosidase, driven by strong promoter PglaA (the glucoamylase gene promoter), had a high expression level in A. niger A-8 (25.02 U/mL). The optimized ATMT system was found to be effective and suitable for A. niger, and should be a useful tool for studying the function of A. niger genes and for industrial breeding of this strain.
- 公益社団法人 日本農芸化学会の論文
著者
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Liu Meng
Key Laboratory Of Digital Earth Center For Earth Observation And Digital Earth:graduate University O
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Li Ming
Key Laboratory Of Animal Ecology And Conservation Biology Institute Of Zoology Chinese Academy Of Sciences
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LIU Meng
Key Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, College of Biotechnology, Tianjin University of Science and Technology
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ZHOU Liying
Key Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, College of Biotechnology, Tianjin University of Science and Technology
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HUANG Yunyan
Key Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, College of Biotechnology, Tianjin University of Science and Technology
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SUN Xin
Key Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, College of Biotechnology, Tianjin University of Science and Technology
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LU Fuping
Key Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, College of Biotechnology, Tianjin University of Science and Technology
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LI Ming
Key Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, College of Biotechnology, Tianjin University of Science and Technology
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