Glycosaminoglycan and Collagen Facilitate the Degradation of Pigment Epithelium-Derived Factor by Chymotrypsin

スポンサーリンク

概要

• 論文の詳細を見る

• Pigment epithelium-derived factor (PEDF) is a member of the serine protease inhibitor family. It is present in a variety of tissues and organs, including plasma. Here, PEDF was purified from human plasma by use of a dermatan sulfate affinity column, and then hydroxyapatite, gel filtration and ion exchange columns. It did not form a complex with various proteases, including chymotrypsin, elastase, kallikrein, thrombin, plasmin, cathepsins G, activated protein C, and urokinase, but collagen type I facilitated the degradation of PEDF by chymotrypsin more than 10-fold. Dermatan sulfate, heparan sulfate, and heparin showed similar effects, but other glycosaminoglycans, such as hyaluronic acid, chondroitin sulfate A, C, D, E, and keratan sulfate, had no effect on PEDF degradation.

• 公益社団法人 日本農芸化学会の論文

著者

• SAITO Akio

  Division of Basic Medicine, Faculty of Medicine, Kinki University

関連論文

• Glycosaminoglycan and Collagen Facilitate the Degradation of Pigment Epithelium-Derived Factor by Chymotrypsin

スポンサーリンク