Hydroxynaphthoic Acids Identified in a High Throughput Screening Potently Ameliorate Endoplasmic Reticulum Stress as Novel Chemical Chaperones

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概要

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• Folding of newly synthesized protein occurs in endoplasmic reticulum (ER) and is assisted by chaperone molecules. In ER stress conditions, misfolded proteins are enriched in a lumen of ER perturbing its normal function, which triggers cellular self-defense mechanism, the unfolded protein response (UPR). It was reported that tunicamycin-induced ER stress can be modulated with high concentration of chemicals such as 4-phenylbutyric acid and salicylate. In search of assay systems to identify such compounds, we have developed a cell-based reporter assay where renilla luciferase activity is driven by glucose-regulated protein 78 (GRP78) promoter. Using our reporter assay, we have screened chemical libraries and found that hydroxynaphthoic acids, especially 1-, 3-, and 6-hydroxy-2-naphthoic acids, potently decrease the ER stress signal, showing an order of magnitude better activity than salicylate. UPR markers such as GRP78, C/EBP homology protein (CHOP) and phosphorylated protein kinase RNA-activated (PKR)-like ER kinase (PERK) were significantly down-regulated with hydroxynaphthoic acids in western blot. Among the analogues, 1-hydroxy-2-naphthoic acid was the most potent in down-regulating those UPR markers. Further, both phosphorylated inositol-requiring enzyme 1α (IRE1α) and spliced form of X-box binding protein 1 (XBP1) were decreased in the protein and the mRNA level, implying both PERK and IRE1α branches in UPR mechanism are controlled with hydroxynaphthoic acids. Taken together, it was suggested that hydroxynaphthoic acids exert their ER stress-reducing activity prior to the UPR activation as chemical chaperones do. In summary, we report a cell-based assay system for the screening of ER stress-reducing compounds and hydroxynaphthoic acids as novel series of chemical chaperones.

著者

• Park Myung-whan

  Gyeonggi Bio-Center (GGBC), Gyeonggi Institute of Science and Technology Promotion

• NAM Tae-Gyu

  Department of Food Science and Biotechnology, Kyung Hee University

• Nam Tae-gyu

  Department of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University

• Jeong Kwi-wan

  Gyeonggi Bio-Center (GGBC), Gyeonggi Institute of Science and Technology Promotion

• Ku Jin-mo

  Gyeonggi Bio-Center (GGBC), Gyeonggi Institute of Science and Technology Promotion

• Park Sun-mi

  Gyeonggi Bio-Center (GGBC), Gyeonggi Institute of Science and Technology Promotion

• Yang Jung-eun

  Gyeonggi Bio-Center (GGBC), Gyeonggi Institute of Science and Technology Promotion

関連論文

• Protective Effect of Detoxified Rhus verniciflua Stokes on Human Keratinocytes and Dermal Fibroblasts against Oxidative Stress and Identification of the Bioactive Phenolics
• Hydroxynaphthoic Acids Identified in a High Throughput Screening Potently Ameliorate Endoplasmic Reticulum Stress as Novel Chemical Chaperones
• Protective Effect of Detoxified Rhus verniciflua Stokes on Human Keratinocytes of the Bioactive Phenolics

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