Simultaneous Determination of Chlorpromazine and Levomepromazine in Human Urine by High-Performance Liquid Chromatography Using Electrochemieal Detector

概要

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A method for the simultaneous determination of chlorpromazine (CPZ) and levomepromazine (LPZ) in human urine has been developed using high-performance liquid chromatography (HPLC) with electrochemical detectlon.<BR>CPZ and LPZ were separated by a reversed phase HPLC on μ Bondapak C18 (4.6mm i.d. ×250mm) using mobile phase of methanol-ethanol-tetrahydrofuran-acetate buffer (pH 5.0)(53.5: 9.0: 2.5: 35.0, v/v), containing 20mM NaClO4, and the separation was completed within 10min.Minimum detectable amounts on this detector were 500Pg for CPZ and 200Pg for LPZ with higher sensitivity than UV detector.Recoveries of CPZ and LPZ (2 and 20μg) from urine were 84.0% and 98.0%, respectively.<BR>This method was apPlied to the determination of CPZ and LPZ concentrations in the urine of schizophrenics receiving simultaneous dose of CPZ and LPZ.
Japan Society of Clinical Chemistryの論文