Studies on the Determination of Serum Glucose with o-Toluidine Using No Glacial Acetic Acid

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概要

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• The determination of serum glucose with o-toluidine using a glacial acetic acid medium is sensitive, precise and accurate enough for the requirements of a clinical laboratory. It is not convenient for a routine work because g acial acetic acid has irritant vapor.<BR>Therefore, a new method of determination of serum glucose with o-toluidine using no glacial acetic acid (TGBM method) is presented. The procedure employs a mixture of glycollic acid, benzyl alcohol and methyl cellosolve instead of glacial acetic acid.<BR>The new reagent (TGBM reagent) is non irritant, colorless, and stable for at least several months when stored at 4° in a brown bottle.<BR>The coefficient of variation was ±2% by the TGBM method; recovery experiments gave a mean yield of 100%. Good agreement was observed between the TGBM method and the UV method using hexokinase-G-6 PDH or OTB method.

• Japan Society of Clinical Chemistryの論文

著者

• 渡辺 富久子

  神戸女子薬科大学

• 山下 真岐子

  神戸女子薬科大学臨床生化学

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