Development of a New Immuno-Gold Staining Method for Fecal Occult Blood Test

概要

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Problems exist for the improvement of the latex agglutination method for fecal occult blood testing. The main problems involve the necessity for the punctuality of reaction time and the need for skills in the judgment of test result.<BR>With the intention of improving on these problems, we developed an immuno-gold staining method for fecal occult blood testing using colloidal gold bound with and human hemoglobin monoclonal antibody and membrane bound with anti human hemoglobin.<BR>When the 0.2-400μg/ml of human hemoglobin were tested by this method, the clear purple color of colloidal gold appeared on the membrane. The reaction was completed within 3min and the result color did not change for 60min.<BR>As far as testing was concerned, the other animal hemoglobins, the food materials and the drugs had no effect on the test result by this method. There was a good correlation between the test results of the 499 samples obtained at hospitals by this method, by the latex agglutination method and by the hemagglutination method.<BR>In the three methods, the test results obtained by this method was correlated very well with the results of endoscope testing. As mentioned above, this method is very well suited for operation and may be used as a routine testing fecal occult blood.
Japan Society of Clinical Chemistryの論文