Zymographical Studies of Lipase and Lipoprotein Lipase:1. Method of Lipase-Zymography

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概要

• 論文の詳細を見る

• A new method was described for the separation of lipases by electrophoresis utilizing a cellulose acetate membrane.<BR>Triolein or partially purified-endogenous fat from obese mice was used as a substrate of lipases and liberated fatty acids from substrate hydrolysed by enzymes on the membrane were coupled with copper ion in a solution of copper-triethanolamine. Coupled-copper ion was stained by diphenylcarbazide.<BR>Four bands were found in the zymograms of lipases in the aqueous extracts from different tissues in mice, which were designated ae Lpl, Lp2, Lp3 and Lp4, respectively.<BR>Furthermore, since fractionated lipoprotein lipase from postheparin plasma by heparin affinnity column chromatography was also observed by zymography, it was known that a new method wight be useful for investigation of lipoprotein lipase.

• Japan Society of Clinical Chemistryの論文

著者

• 楠見 博明

  和歌山県立医科大学腎センター準備室

関連論文

• Zymographical Studies of Lipase and Lipoprotein Lipase:1. Method of Lipase-Zymography
• Zymographical Studies of Lipase and Lipoprotein Lipase (LPL).:2.Heparin-Releasable LPL

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