Determination of Vanilmandelic Acid in Urine by High-Performance Liquid Chromatography with Ultraviolet Detection

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We developed a high-performance liquid chromatographic method with ultraviolet detection using a column-switching system for the determination of vanilmandelic acid (VMA levels in human urine. The lower detection limit of VMA in urine was 0.5μg/ml. VMA in sample solutions was stable for at least 25 h at room temperature under a room light.<BR>The recovery of VMA in urine was 92% over the range of 0.50 to 5.03μg/0.5ml urine. Reproducibilities (CV) for within- and betweenassays of samples containing three concentrations of VMA (low, medium and high) were 1.3%, 1.2% and 1.1% (n=10 each), and 5.0%, 3.1%, and 1.9% (n= 5 each), respectively. The VMA levels in urine measured by the present method (y) correlated well with those by a conventional method (x) r = 0.92, y 0.72x+0.19, n= 41).