Study of a Liquid Type Reagent for Determination of Uric Acid by Using Thermostable Recombinant Uricase

概要

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The use of liquid type reagent is required in common use at clinical laboratories to keep more precise and accurate quality control. However, there are several problems concerning to the raw materials of reagents which contains enzymes, coenzymes and substrates etc. Because these are unstable in liquid solution for longterm storage. For the measurement of uric acid, we developed new thermostable recombinant uricase from Bacillus sp., TB9O strain. Although uricase from Candida sp. has been currently used for the determination of uric acid, the enzyme activity in a liquid solution is unstable for long-term storage. A recombinant enzyme from Bacillus sp., TB9O strain has two excellent properties, which make it superior to that from Candida sp.;the enzyme activity in a liquid solution has been kept for more than one year under the storage condition of 10°C, the enzyme has a broad optimum pH range. It is a suitable liquid reagent for the determination of uric acid. We evaluated a liquid type reagent using recombinant type enzyme for the determination of uric acid. A good linearity, precision and no influences of interfering substances were obtained. This liquid reagent with using a recombinant uricase is useful for the routine test at clinical laboratories.
Japan Society of Clinical Chemistryの論文