Estimation of Complete Saponification Conditions in the Abell-Kendall Method by High Performance Liquid Chromatography

概要

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Incomplete saponification of cholesterol esters causes a systematic error in cholesterol measurement by the Abell-Kendall method. The saponification of cholesterol esters; linoleate, oleate, palmitate, arachidonate and stearate, which occupy >1% of all choresterol esters in serum was examined by high performance liquid chromatgraphy to clarify the condition of complete (>99.9%) saponification. The cholesterol esters corresponding to 0.8% of total cholesterol remained unsaponified after 60 min at 37°C but 99.9 % were saponified after 90 min.
Japan Society of Clinical Chemistryの論文