Comparative Studies on Cholinesterase Activities in Human and Animal Sera

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Cholinesterase activities (ChE) with various substrates and in the presence and absence of inhibitors were measured in sera from human and animals, and the following results were obtained. <BR>1. True ChE, which is inhibited by caffeine but not ethopropazine with acetylthiocholine as substrate, was present to a greater extent in animals than in human. Differences of the inhibition by caffeine and ethopropazine suggest that proportions of true ChE to total ChE were about 60% in rabbits, 40% in male rats, 16% in dogs, 10% in monkeys and 7% in human. <BR>2. In rabbits, some (type I) hydrolysed butyrylthiocholine to a significantly higher extent than others (type II). Since this hydrolysis was mostly inhibited by DFP but hardly inhibited by EDTA or eserine, it was probably due to carboxyesterase. <BR>3. In female rats, ChE with acetylthiocholine as substrate showed age-dependent differences in activity, Km value, and inhibtions by caffeine and ethopropazine. True ChE was constant regardless of age, indicating that the differences were due to the change of pseudo-ChE.
Japan Society of Clinical Chemistryの論文