A CONTRACTILE PROTEIN FROM THE INTESTINAL WALL

概要

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1) A contractile protein extracted from the intestinal wall showed superprecipitation at low ionic strength in the presence of Mg++ and ATP and it possessed ATPase activity (E. C. 3. 6. 1. 3.). Superprecipitation of the protein was enhanced by an increase of Mg++ concentration up to 20mM in a medium and was closely associated with enhancement of ATPase activity of the protein as Mg++ concentration increased. 2) The electronmicrogram of the protein showed, at high ionic strength without ATP, an "arrow head" structure characteristic of myosin B from skeletal muscles. This structure disappeared after addition of ATP. 3) It might be concluded that a contractile protein, the so-called actomyosin was observed in the intestinal wall.
Japan Society of Clinical Chemistryの論文