Modified Assay Method for Placental Alkaline Phosphatase:The Method of Electrophoresis and Heat Treatment

概要

論文の詳細を見る
1. A method was developed for the separative assay of placental alkaline phosphatase in human serum from the isozymes derived from other organs using acetate membrane electrophoresis with an electrophoresis buffer containing 2×10-3M of MgCl2. 2. the activity of placental alkaline phosphatase was completely recovered after the electrophoresis for 40 minutes by the addition of 2×10-3M of MgCl2 to the electrophoresis buffer and to the applied serum. 3. It was possible to measure accurately the placental alkaline phosphatase activity between l and 30 K-AU. using this method. 4. Neale1) described that placental alkaline phosphatase activity could be determined by treating serum a 56° for 30 minutes. But it is difficalt to obtain the constant results, because it is essential to adjust the temperature accurately at 56° 5. Placental alkaline phosphatase actMty in sera could be correctly determined only when the samples were heated at 56-58° for 30 minutes after the treatment with 10-3M of EDTA and 3×10-3M of MgCl2 at 37° for 20 minutes.